This Error would be occurred by some of Following reasons.
1) The message is saying that your configuration file is corrupt in some way. However it also says that it can't actually access the config file. So I'd ignore the original message about corruption/lack of validity as this is most likely just the effect of not being able to read the file due to a lack of authorization.
The reason it cannot read the config file is because the process running your web app does not have permission to access the file/directory. So you need to give the process running your web app those permissions.
The access rights should be fairly straightforward, i.e. at least Read, and, depending on your app, maybe Write.
Above, you mention IUSR etc. not being in the properties for web.config. If by that you mean that IUSR is not listed in the security tab of the file then it's a good thing. One doesn't want to give IUSR any kind of permission to web.config. The role IUSR is an anonymous internet user.
The file web.config should only be accessible through your application.
The problem is you haven't said which OS and IIS version you are using so it's difficult to advise which steps to take.
I.e. in IIS 7.5, the error message you're quoting is likely to occur due to your ApplicationPoolIdentity not being assigned the permissions. Your web application belongs to an application pool and so you need to give the permissions to the OS account that your web application's application pool runs under. Often this is something like NetworkService but you may have customized it to run under a purpose made account. Without more info it's difficult to help you.
2)The asp.net account not appear in the IIS manager because I didn’t check its check Box in IIS to do this in windows 7 follow the steps
Open control panel
Click on” program” link (not uninstall programs)
Click” turn windows features on/off” link
locate” Internet Information services IIS” in the pop up window and expand its node
Expand the” World Wide Web Service” node
Expand “Application Development Features” node
check the check box of”ASP.NET”
Then click ok button
Now you will see the Asp.net account on the IIS manager and by default you see IIS account Now you should move you asp.net website from “my document” to another place the IIS have permission to access it (to any partition on your computer) Now browse your website from IIS manager and it should work.
3) You need to assign permissions for IIS_IUSRS on the local machine (but you don't have to assign for IUSR, in fact it will work even if you explicitly deny permissions).
To assign permissions, just right click on the folder and on the security tab make sure to grant the correct permissions, and if the user is not listed then click "ADD", and enter IIS_IUSRS (and make sure that under "domain" the local computer is selected, or enter in the name field YourLocalComputerName\IIS_IUSRS), and then you are good to go.
If you want you can instead of assigning permissions to the IIS_IUSRS group, you can instead assign to the app pool which should in general be "IIS APPPOOL\ app pool name".
4) Make sure you have the application pool set to the correct version of the framework. You'll also need to make sure your aspnet or IUSR users have read access to the application's directory.
5) I too had the similar issue and i fixed it by commenting some sections in web.config file.
The project was earlier built and deployed in .Net 2.0. After migrating to .Net 3.5, it started throwing the exception.
Resolutions:
If your configuration file contains "<sectionGroup name="system.web.extensions>", comment it and run as this section is already available under Machine.config.
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